BIOCHEMICAL TECHNIQUES-COLORIMETRY-OPTICAL DENSITY?

A 4ml solution of a protein whose concentration is 20micrograms gives an O.D of 0.5.what will be the O.D of 8ml of the same solution?

Comments

  • if 20 microgram is the conc. of stock slution then OD will be same whether u take 4 ml or 8 ml. but if the conc. is 4 micrograms /ml it will chane and wil have to be determined colorimetrically.

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